Figure 7.

Overexpression of mGPDH favors metabolism of dihydroxyacetone to pyruvate and lactate rather than glucose. Mouse hepatocytes were either untreated or treated for overexpression of mGPDH (L and H) as in Figs. 4 and 5. After a 20-h culture, they were incubated for 2 h in glucose-free DMEM containing either 5 mm DHA (A–F) or 5 mm DHA and 0.125 mm octanoate (G–L) without (open bar) or with (shaded bar) 200 μm AOA. A and G, cell G3P; B and H, glucose production; C and I, pyruvate + lactate; D and J, total production of glucose + pyruvate + lactate expressed as C3 units; E and K, glucose percentage of total metabolism; F and L, lactate/pyruvate ratio. Results are means ± S.D. (error bars) for n = 11–12 (A–F) or n = 3–5 (G–L). *, p < 0.05, effect of mGPDH overexpression; $, p < 0.05, effect of AOA.