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. 2018 Dec 31;294(8):2606–2615. doi: 10.1074/jbc.RA118.004538

Figure 2.

Figure 2.

Serum half-lives of rh proSP-C and Bri2 BRICHOS after systemic injection. A–E, representative decay curves and Western blots of individual mice for rh proSP-C BRICHOS (A), the rh Bri2 BRICHOS-AU1 mixture (B), and isolated rh Bri2 BRICHOS-AU1 monomers (C), dimers (D), and oligomers (E). The half-lives were determined by densitometry of Western blot bands corresponding to the monomers (indicated by arrows). Bands corresponding to BRICHOS dimers and oligomers, which resist complete reduction, in particular for oligomers of rh Bri2 BRICHOS-AU1, were observed. A nonspecific band between 25–35 kDa of unknown origin was observed in B, D, and E. Western blot analysis was performed using an anti-S tag and anti-AU1 tag for the detection of rh proSP-C and Bri2 BRICHOS, respectively. The time points above the gels refer to minutes after injection, and lanes marked with + refer to samples from mice injected with 20 mg/kg rh BRICHOS; − denotes controls injected with PBS. F, summary of rh BRICHOS half-lives from two (rh Bri2 BRICHOS-AU1 dimers) or three (all other groups) different mice. The error bars show mean values and standard deviations. **, p < 0.001; ***, p < 0.0001 versus proSP-C BRICHOS.