Figure 6.

Rh Bri2 BRICHOS-AU1 is found in the plexus choroideus, cortex, and striatum after systemic injection. A–P, the lateral ventricle and surrounding tissue (A–D), cortical coronal sections (E–L), the striatum area (M–O), and fimbria/thalamic area (P) collected 1 or 2 h after injection of rh Bri2 BRICHOS-AU1 mixture (20 mg/kg) (B–D, G, H, K, L, and N–P) or PBS (A, E, F, I, J, and M). Tissues in A–H and M–P were stained with a mouse anti-AU1 antibody, followed by a secondary HRP-conjugated antibody and development with DAB staining. Tissues in I–L were stained with a rabbit anti-AU1 antibody followed by an AP-conjugated secondary antibody and development with permanent AP red solution. All samples were counterstained with hematoxylin. The arrows and boxes highlight areas with positive staining. cp, choroid plexus; lv, lateral ventricle; csf, cerebrospinal fluid; fi, fimbria; hs, hippocampal sulcus; th, thalamus. Scale bars = 50 μm in A–H and M–P and 25 μm in I–L.