Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 20;294(8):2924–2934. doi: 10.1074/jbc.RA118.005471

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Sulistomo et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 8. — Effects of Fhod3 depletion in morphological segmentation of rhombomeres. A, schematic representation of the orientation of optical sectioning of whole-mounted neural tubes embedded in an agarose gel used in B. B, orthogonal projection of Z-stack slices of whole-mounted neural tubes at rhombomere 4 in the hindbrain of Fhod3^+/+ (+/+) and Fhod3^−/− (−/−) embryos at E9.5. Whole-mount embryos were subjected to immunofluorescence staining for ZO-1 (green) and F-actin (red) (B, boundary). Scale bars, 100 μm. C, schematic representation of the orientation of longitudinal coronal sections used in D. D, longitudinal coronal sections of the hindbrain of Fhod3^+/+ and Fhod3^−/− embryos at E9.5. Sections were subjected to fluorescence staining for F-actin (red) and nucleus (blue). Inter-rhombomere boundaries are indicated by arrowheads. Scale bars, 100 μm. E and F, whole-mount images of Fhod3^+/+ (E) and Fhod3^−/− (F) embryos at E9.5 in lateral and dorsal views (left and right, respectively). Inter-rhombomere boundaries and rhombomere bulges are indicated by arrowheads and arrows, respectively. Scale bars, 500 μm.