Fig. 7.

Reduction of 488- and 405-nm evoked photocurrents from ChR2 C128A/H134R/T159C, CoChR C108S, and CoChR C108S/D136A channels. (a) Effect of coillumination of different 594-nm laser powers on 488 nm (top) or 405 nm (bottom) evoked currents; 594-nm laser powers are indicated in the color-coded inset and were tested in 10% steps from 0% to 100% laser power. (b) Relationship between 594-nm laser intensity and photocurrent reduction. (c) Influence of activating 488 nm (top) or 405 nm (bottom) laser power on 594 nm light-mediated photocurrent reduction. Scale bars indicate recorded currents (vertical) and time (horizontal). (d) Photocurrent reduction of 488-nm light (top) and 405-nm light (bottom) evoked currents in CoChR C108S (left) and C108S/D136A (right) mutants. Trace and stimulation bar colors represent laser wavelengths and intensities (5%, 10%, 15%, and 20%).