Figure 2.

BBB permeability evaluation of diabetic Lepr^db/db and control mice, determined by whole brain lysate spectrophotometric measurements of Evans blue (EB) dye. (a) Representative images of whole brains, brain coronal views and kidneys, following EB challenges. (b) Quantification of EB extravasation by spectrophotometric measurements of whole brain lysates. 8–10 weeks-old diabetic Lepr^db/db and control littermate mice were tested (n = 4. ns – non significant, P = 0.811 unpaired two-tailed Student’s t-test, all data are mean ± s.e.m).