Figure 4.
In Vivo Expression of EGFP and AsRED following Subretinal Administration of AAV/miR(5,B,7)-AsR-PE, Assessed 57 dpi
Mice were injected with 4.2 × 109 vg AAV/miR(Irr)-AsR-PE (A–C), AAV/miR(5,B,7)-AsR-PE (D–F), and AAV/miR(5,B,7)-PEDF-PE (G–I). 57-dpi mice were euthanized and eyes were enucleated. Following removal of the neuroretina from the eye cup, the RPE cell layer was exposed, and flat-mounts were prepared as described in the Materials and Methods. Representative images of flat-mounts from each group of injections showing whether EGFP (green) and AsRED (red) are expressed in vivo were obtained using fluorescence microscopy. Combined images (C, F, and I) were obtained by merging the respective images shown in (A and B), (D and E), and (G and H). Scale bar, 50 μm AsRED (AsR), red fluorescent marker protein; vg, vector genomes.