Figure 1.

PPFIA1, a Direct Target Gene of miR-181a in CML

(A) miR-181a was downregulated in CML cells. The qPCR analysis of miR-181a in normal human PBMCs, human CML samples, and CML cell lines. (B) PPFIA1 mRNA was upregulated in CML cells. The qPCR analysis of PPFIA1 mRNA in normal human PBMCs, human CML samples, and CML cells. (C) Experimental design for microarray analysis or SILAC-LC-MS/MS analysis. (D) 15 candidate target genes of miR-181a were identified by combining miRNA predictions, miR-181a transfection microarray, and SILAC-LC-MS/MS analysis. (E) Heatmap showed the different expression of 15 candidate target genes in miR-181a transfection microarray. (F) PPFIA1-siRNA and miR-181a mimics (100 nM) inhibited the mRNA and protein expression levels of PPFIA1. (G) Human PPFIA1-3′ UTR and its miR-181a target site predicted by TargetScan software. miR-181a-directed repression of Renilla luciferase reporter genes containing PPFIA1-3′ UTR segments. The data were presented as the mean ± SD obtained from at least three independent experiments. Significance was determined by Student’s t test, *p < 0.05 versus NC control. Mut, contains 7-base mutation at the miR-181a target seed region.