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. 2019 Feb 8;16:1–14. doi: 10.1016/j.omtn.2019.01.015

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

PARP1, an Important Downstream Molecule of PPFIA1 and BCR/ABL

(A) K562 or K562-Flag PPFIA1 cells were used to perform BCR/FLAG IP assays. The IP products from BCR and FLAG affinity purification were separated by SDS-PAGE and analyzed by mass spectrometry. The PARP1 was both related to exogenous PPFIA1 and endogenous BCR in K562 cells. (B) The molecular docking of PPFIA1 with PARP1 by using Discovery Studio 4.5. The molecular docking of ABL1-PARP1 (C) or F-actin-PARP1 (D) by using Discovery Studio 4.5. (E) The binding analysis of F-actin-PARP1 was performed in surface plasmon resonance imaging (SPRi) binding assay. (F) FLAG-immunoprecipitates were immunoblotted with anti-Flag and anti-PARP1. PARP1 was immunoprecipitated with FLAG affinity purification. (G) PARP1-immunoprecipitates were immunoblotted with anti-PPFIA1 and anti-PARP1. PPFIA1 was immunoprecipitated with anti-PARP1. (H) ABL1-immunoprecipitates were immunoblotted with anti-ABL1 and anti-PARP1. PARP1 was immunoprecipitated with anti-ABL1. (I) PARP1-immunoprecipitates were immunoblotted with anti-ABL1 and anti-PARP1. ABL1 was immunoprecipitated with anti-PARP1.