Figure 1.
Production of 3-HFA-TAG with RcGPAT9, RcLPAT2, and RcPDAT1A coexpression. Representation of the incorporation of HFA at the sn-1, -2, and -3 positions of TAG precursors is shown. Blue arrows show the assembly of 18:1-PC substrate for RcFAH12. Orange arrows show the flux of HFA out of PC and onto TAG. Red arrows show the direction of glycerolipid assembly going from G-3-P to TAG. Castor enzymes coexpressed with RcFAH12 are shown in red. Lipids are G-3-P, LPA, DAG, TAG, PC, and lysophosphatidylcholine (LPC). Enzymes are FAH12, GPAT9, LPAT2, and PDAT1A.