Efficient incorporation of HFA produces 3-HFA-TAG. A, Percentage contribution of each HFA-TAG species to total TAG. B, Stereochemical position of HFA in 1-HFA-TAG. Lines CL37 (gray), PD (red), PD_L2 (green), and PD_G9_L2 (blue) are shown. Error bars represent sd; n = 3 independent replicates of pooled seed taken from 30 siliques of 10 plants. Statistical analysis: one-way ANOVA with posthoc Tukey’s test. Data marked with different letters are statistically different (P < 0.001).