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. 2019 Jan 14;179(3):1001–1012. doi: 10.1104/pp.18.01218

Figure 6.

Figure 6.

Cross test of C1 and P65/P50 split TALE scaffolds. The constructs used are visualized by schematic representations. The diagram shows the results of the GUS-assay after Agrobacterium-mediated transient expression of indicated split-TALE components (BC, AC) and the reporter construct (4×STAP-Ω-GUS-GFP) in N. benthamiana leaves. The color code indicates corresponding split-TALE constructs (C1, green boxes; P65/P50, blue boxes) or free GFP (35S::GFP, white boxes). Free GFP was used to monitor background activity of the single BCs and ACs, respectively. 35S::GUS serves as the positive control. White bars are for assays where only one component of the sTALE, either BC or AC, was expressed. Black bars are for assays where both functional components were expressed. The identity of the expressed component is indicated below the graph by green boxes or squares. Split-TALE activity was only measurable in the presence of the matching IDs. Error bars represent sd (sd) of three biological replicates. Asterisks indicate a significant difference in activity of the same BC tested with free GFP (white box; Student’s t test; **P-value ≤ 0.01, ***P-value ≤ 0.001). Experiments were performed two times with similar results. All raw data are available in the Supplemental Data.