Skip to main content
. 2019 Jan 24;179(3):943–957. doi: 10.1104/pp.18.01220

Figure 6.

Figure 6.

An example of in vivo validation of two MoChlo-enabled constructs in potato chloroplasts. A, Schematic representation of test operons controlled by two examples of promoter::5ʹUTR combinations. The operon (red) is controlled by the rrn promoter (P1) along with a synthetic RBS as 5ʹUTR (5ʹ1; blue and gray; Operon X) or the promoter::5ʹUTR fusion of accD gene (P2:5ʹ2; blue and gray; Operon Y). The expression of mEmerald (mEme; green) is under the control of the operon promoter, while the selection gene, aadA (yellow), is under the control of both promoter::5ʹUTR (P-5ʹ[3]; blue and gray) and terminator/3ʹUTR (T; black) of the psbA gene. B to I, Confocal images of transplastomic green callus transformed with Operon X (B–E) or Operon Y (F–I). In both cases, the mEmerald fluorescent signal is localized to chloroplasts. B and F show mEmerald signal; C and G show chlorophyll autofluorescence; D and H show bright-field images; and E and I show merged images. Bars = 20 µm for all confocal images.