Figure 8.

Schematic of the MoChlo kit for combinatorial assembly of operons for metabolic engineering. In the current iteration of the kit, combinatorial assembly of individual cassettes (Level-1 constructs) can be conducted using 47 promoters, 38 5ʹUTRs, nine promoter:5ʹUTR fusions, and 10 terminators/3ʹUTRs. In addition, the user has the ability to assemble up to seven individual cassettes, each with its own regulatory elements and GOIs. Furthermore, during Level-2 assembly, the user can choose from three different integration sites (trnI-trnA, trnV-3ʹrps12, and trnG-trnfM) from three different plant species (tobacco, maize, and potato). After completing assembly, the user would then bombard the Level-2 constructs into leaf discs and select and regenerate transplastomic plants.