Figure 5.

Salidroside is the bioactive compound of R. idaeus (var Prestige) against HD. S. cerevisiae W303-1A cells either expressing HTTpQ103-GFP or harboring the empty vector (control) were pregrown in SC raffinose medium. A, Growth curves of cells incubated in SC Gal medium for 24 h (left). The confidence interval for the maximum growth rate (μ) and final biomass is shown on the right. B, Cell growth assessed after 6 h induction in SC medium with Gal by phenotypic growth assays in SC medium with Glc, Gal, or glycerol. C, Superoxide production after 18 h induction in SC medium with Gal was determined by flow cytometry. D, Growth of the S. cerevisiae BY4741 wild type and sod1 mutant strains in the presence of H₂O₂ and/or salidroside in SC Glc medium for 24 h. Representative images are shown and the values in all graphs represent the mean ± se of at least three biological replicates. Statistical differences are denoted as *P < 0.05, **P < 0.01, and ***P < 0.001, relative to that in the HTTpQ103-GFP condition (C) or in the untreated condition (D). MFI, median fluorescence intensity; a.u., arbitrary units.