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Enzymatic activity of GGPS11, PSY, and translational fusion proteins in E. coli. GGPS11 (GGPS), PSY, and fusion proteins GGPS11-PSY (GGPY) and PSY-GGPS11 (PYGG) in various combinations were coexpressed with CrtI in E. coli cells. A, Lycopene amounts; acetone extracts are shown below. B, Immunoblot with 60 µg bacterial lysates using anti-PSY antibodies. GGPS11 alone was included as a negative control. Data are means ± SEM of three replicate experiments.
