Figure 6.

Complementation of mutated chimeric PSY-GGPS proteins. A and B, Recombinant PYGG was incubated with 20 µm DMAPP/20 µm IPP for 1 h, then amounts of GGPP and phytoene were determined and expressed in nmol IPP equivalents. A, Assay of PYGG fusion protein and versions with impaired PSY (pyGG1 and pyGG2) complemented with wild-type PSY (+PSY). B, Assay of PYGG fusion protein and versions with impaired GGPS11 (PYgg1 and PYgg2) complemented with wild-type GGPS11 (+GGPS). C to E, Standard enzyme assay with equimolar concentrations of GGPS11 and PSY (C), PYGG (D) and GGPS11 and PSY in a molar ratio of 1:10 (E). All assays were performed with 138 nm PSY and PYGG and 20 µm DMAPP/20 µm IPP. Data are means of three replicate experiments.