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. 2018 Nov 30;67(3):203–219. doi: 10.1369/0022155418815860

Figure 3.

Figure 3.

Changes occurred in other SHexCer species located in the white matter but phospholipid species localized in gray matter regions did not differ significantly either in the Shi or Cz-fed mouse. (A) The normalized intensity of SHexCer (t36:1) was elevated; whereas, normalized intensity of SHexCer (t42:2) was reduced in the CC of Shi mouse compared with the WT. However, these differences were not statistically significant. PC shown here as well as phospholipid species that belong to PI, PE, and PA did not differ significantly between the Shi and WT mouse. Additional species belonging to these lipid types are shown in the supplementary information. (B) There was a significant rise in the normalized intensity of SHexCer (t36:1) and significant decrease in the normalized intensity of SHexCer (t42:2) in the CC of Cz-fed mouse at 6w. By 12w, the intensities of both lipid species restored. However, the intensity of SHexCer (t42:2) remained significantly lower compared with the CD-fed mouse. Like observed in the Shi mouse, the normalized intensities of PC, PI, PE, and PA species did not differ significantly between the Cz-fed and CD-fed mouse at 6w and 12w. Additional species of the above listed lipid types are shown in the supplementary information. Unpaired two-tailed t-test or two-way ANOVA. Differences were considered as significant when p<0.05. Abbreviations: Shi, shiverer; Cz, cuprizone; SHexCer, sulfatide; CC, corpus callosum; WT, wild type; PC, phosphatidylcholine; PI, phosphatidylinositol; PE, phosphatidylethanolamine; PA, phosphatidic acid; CD, chow diet; w, weeks.