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. 2018 Dec 21;47(1):433–440. doi: 10.1042/BST20180316

Figure 1. Functional UapA dimer formation is dependent on membrane lipids.

Figure 1.

(A) UapA in detergent-based solution exists normally as a dimer in complex with membrane lipids as determined by native MS. Delipidation results in dissociation of the UapA dimers into the monomeric form. Dimers can be recovered by the addition of PI and PE both alone and in combination. (B) A potential lipid-binding site, comprising R287, R478 and R479, at the interface between the two protomers, was identified by molecular dynamics (MD) simulations. Mutation of all three Arg residues to Ala results in a loss of UapA transport function in the native host, Aspergillus nidulans. This triple mutant isolates almost exclusively in the monomeric form.