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PD-L1 was overexpression in mammosphere cells of breast cancer. (a) Compared with adherent cells, the expression of PD-L1 mRNA in mammosphere of MCF-7 was increased. (b) Compared with adherent cells, the expression of PD-L1 mRNA in mammosphere of MDA-MB-231 was overexpressed. (c) The protein level of PD-L1 in BCSCs derived from microspheres significantly increased compared with adherent cells. ∗∗∗ P < 0.001; compared with the adherent cells.
