Fig. 2.

Modulation of VEGFR2 expression changes the growth and metastasis of gastric cancer cells. a & b Real-time PCR and Western blot analysis of relative levels of VEGFR2 in gastric cancer cell lines and GES-1 cells. c & d Confirmation of VEGFR2 knockdown in MKN-45 cells by using Real-time PCR and Western blot analysis. e The effect of VEFR2 silencing on the invasion of MKN-45 cells. f The effect of VEFR2 knockdown on the growth of MKN-45 cells in vitro. g Optical images of MKN-45 xenograft tumors. Tumors were dissected out 20 days after cell injection. h Tumor weight of MKN-45 xenograft tumors. i The effect of different doses of Apatinib (Apa) on the growth of MKN-45 cells in vitro. j Optical images of MKN-45 xenograft tumors after treatment with Apatinib (10 mg/kg). k Tumor weight of MKN-45 xenograft tumors after treatment with Apatinib. l & m Confirmation of VEGFR2 overexpression in SCH cells by using Real-time PCR and Western blot analysis. n The effect of VEFR2 overexpression on the invasion of SCH cells. o The effect of VEFR2 overexpression on the growth of SCH cells in vitro. p Optical images of inoculated SCH tumors. Tumors were dissected out 20 days after cell injection. q Tumor weight of inoculated SCH tumors. *P < 0.05, **P < 0.01, significant differences vs. the respective control groups