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. 2019 Feb 28;17:62. doi: 10.1186/s12967-019-1821-7

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© The Author(s) 2019, corrected publication 2021

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Testosterone induces renal TECs death through the caspase-independent pathway. a Levels of the BNIP3, Bcl-2, Bax pro-caspase-9, caspase-9, pro-caspase-8, cleaved-caspase-8, cleaved-caspase-3 and cytochrome C proteins in HK-2 and TCMK-1 cells transfected with or without the BNIP3 siRNA and stimulated with testosterone (20 nM) were examined by western blotting. b The ΔΨm was detected by determining the ratio of green/red fluorescence of JC-1. Data are presented as values for each group (mean ± SD)