Fig. 8.

ORC1 was a target of miR-140-5p and is suppressed by XIST inhibition. a Sequence alignment of miR-140-5p with the putative binding sites with in the wild-type regions of ORC1. b Targeting relationship between miR-140-5p and ORC1 in Hela cells was confirmed by dual-luciferase reporter assay. c Expression of ORC1 was determined by western blot in Hela and C3AA cells transfected with miR-140-5p mimics or XIST or miR-140-5p mimics + XIST. d Quantitation of the ORC1/GRAPH ratios in Hela and C3AA cells. The western blot and qRT-PCR results manifested that XIST promoted ORC1 expression which was inhibited by miR-140-5p. e The expression of ORC1 was observably upregulated in 30 pairs of cervical cancer tissues (T) compared with adjacent non-tumor tissues (N) determined by qRT-PCR. “n” indicates sample number. f The expression of ORTC1 was examined by qRT-PCR in three cervical cancer cell lines (HeLa, C3AA) and human cervical epithelial cell line HcerEpic **P < 0.01, compared with miR-NC/NC/HcerEpic