Fig. 3.

Steps in Tissue Culture Protocol 1. a Initial cut on 4 day old seedling. b Bisected shoot starting material (cotyledons cut off). c Shoot bisection starting material. d Co-cultivation with filter paper on media. e Inserting half of explant into agar so the meristem contacts the PPT medium. f Untransformed controls and g Explants transformed with construct after 2 rounds of selection of 15 days each. h Transfer to shoot development media. I, J Shoots arising from h. k Intact plants in magenta box or l Test tube. m, n Regenerated plants ready for transfer into soil