Table 1.

Skin thickness measurement of rats and their comparison within groups

Treatments	Before applying DNCB	After 24 h	After 48 h	After 72 h
Sterile water for injection + acetone	2 ± 0.14	2.1 ± 0.0	2.2 ± 0.014	2.3 ± 0.014
Methanol	2 ± 0.01	2 ± 0.02	1.9 ± 0.02	2 ± 0.01
Methanol extract	1.9 ± 0.01	2.9 ± 0.03**++†	2.8 ± 0.01††	2.7 ± 0.07†
n-hexane extract	2 ± 0.16	2.7 ± 0.08**+++†††	2.6 ± 0.01*+††	2.5 ± 0.02**+++
Chloroform extract	1.9 ± 0.11	2.7 ± 0.04†	2.6 ± 0.04†	2.6 ± 0.007†††
Immunomodulator	1.9 ± 0.02	3 ± 0.11**++†	3.7 ± 0.04**++††	3.2 ± 0.12*++†††

All values are mean ± SD

*P < 0.05, **P < 0.01, ***P < 0.001, when other groups were compared with group of rats administered with sterile water for injection + acetone, was compared with control

+P < 0.05, ++P < 0.01, +++P < 0.001, when other groups were compared with group of rats administered with extracts of the test drug

†P < 0.05, ††P < 0.01, †††P < 0.001, when other groups were compared with group of rats administered with sterile water for injection + DNCB