Table 1.

Outer membrane proteins affected by SurA depletion.

Function	Gene name	Ratio surA/WT	β-strands	PDB ID**
Type V secretion	PA14_32780	<0.01	16*
	PA14_32790	<0.01	–
	PA14_61190	0.23	16*
	PlpD	<0.01	16	5F4A, 5FQU
	AaaA (PA14_04290)	<0.01	12*
	EprS (PA14_18630)	0.04	12*
	EstA	0.20	12	3KVN
Siderophore receptors and other TonB-dependent receptors	FpvA	<0.01	22	2W75, 2W16
	FecA	<0.01	22	1PO0, 1PO3
	FiuA	0.04	22*
	PA14_34990	<0.01	22*
	PA14_54180	<0.01	22*
	PA14_26420	0.02	22*
BAM-complex	BamD/ComL	0.30	–
	BamA	0.31	16	4C4V
	BamE/OmlA	0.31	–
	BamB	0.35	–
	BamC (PA14_51260)	0.84	–
Porins	OpdO	<0.01	18	2Y0K, 2Y06
	OpdN	<0.01	18	4FSO
	OprG	0.07	8	2X27
	OprE	0.11	18*
Porins	OpdP	0.13	18	3SYB
	OprD	0.14	18	3SY7
	OprB	0.22	16	4GY, 4GF
	OprQ	0.25	22*
	OprC	0.28	22*
	OprH	0.32	8	2LHF
	OpdC (PA14_02020)	0.35	18	3SY9
	OprF	0.47	8	4RLC
	PA14_31680	0.55	–
	OprM	1.52	4	3D5K
	OpmB (PA14_31920)	1.88	4*
	OpmG	7.37	4*
LPS bio-synthesis	LptD	0.32	26	5IVA
	LptE	0.38	–
T3SS	ExsB (PA14 42400)	<0.01	–
Others	Gbt	<0.01	4*
	FadL (PA14_60730)	<0.01	14	3DWO
	PA14_13130	0.03	–
	PA14_24360	0.04	–
	PA14_36020	7.28	–
	FusA (PA14_13520)	>20.40	4*

OM fractions of PA14 WT and the conditional surA mutant derived from three independent experiments were analyzed by mass spectrometry. Table depicts proteins which are described to be located in the OM and are significantly reduced or increased due to SurA depletion. Multiple t-testing was performed. Significant differences (FDR <0.1) are shown in bold face. Number of β-strands of β-barrel proteins is indicated.

^*Predicted with Boctopus (Hayat and Elofsson, 2012);

^**Accession number of protein data bank (www.rcsb.org) of indicated proteins or orthologs.