Figure 1.

OpiA and related bacterial proteins share conserved motifs with eukaryotic PI kinases.

(A) Overview of conserved sequence motifs found in OFPs. Graphical representations of OpiA orthologous proteins from F. tularensis subsp novicida, tularensis and holarctica, and the Legionella pneumophila OFP LegA5. Conserved residues (black), portions of the gene encode by a separate ORF (blue) and ankyrin repeats in LegA5 (dark grey) are highlighted. Sequence logo indicates conserved catalytic motifs derived from an alignment of OFP sequences from bacterial species representative of the genera shown in panel B (alignment shown in Figure S1).

(B) Bacterial genera found to contain OFPs, grouped by class and phylum and with lifestyle indicated by box colors (eukaryotic-associated, purple; pathogen, grey; other, green).

(C) Alignment depicting the residues shared between OFPs and the indicated human PI3K and PI4K enzymes (Bact, bacterial; Euk, eukaryotic).