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. 2019 Feb 21;10:275. doi: 10.3389/fimmu.2019.00275

Figure 2.

Figure 2

HCQ-mediated inhibition of IFNα production from pDCs in response to CpG-A and ssRNA, but not to R848, can be detected after isolation of PBMC from whole blood pre-treated with HCQ. Whole blood samples from healthy donors were treated with HCQ (1000 ng/ml) or not for 1 h prior to PBMC isolation and then stimulated with CpG-A (10 μM), R848 (1 μM) or ssRNA (4 μg/ml) for 6h. IFNα was analyzed by intracellular cytokine staining. (A) Representative dot plots of IFNα+ cells within a BDCA4+ and CD123+ gate. (B) Percentages of IFNα-producing pDCs detected as shown in A) after CpG-A stimulation (n = 5 donors), R848 stimulation (n = 3 donors), or ssRNA (n = 5 donors) from PBMC isolated from whole blood pre-treated with HCQ or not. Statistical significance was assessed using two-tailed paired Student's t-test (*p < 0.05, ****p < 0.0001).