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. 2018 Nov 14;68(2):331–340. doi: 10.1007/s00262-018-2274-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Schema for WT1 tetramer and intracellular cytokine assays. PBMC samples were divided in two, and one part was used for the WT1 tetramer assay. The other part was cultured in the presence of WT1 HLA class I and II peptides, and IL-2 for 1 week and then used for the WT1 tetramer and intracellular cytokine assays after re-stimulation with each of the WT1 peptides