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. 2019 Feb 15;36(4):51. doi: 10.1007/s11095-019-2586-7

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Partition of rmHSP between aggregated and monomer fractions analyzed using western blot. mAb1 and mAb2 were aggregated using thermal and shaking stresses, respectively. rmHSP70 was added at 0.1% by mass to monomer and aggregated mAbs immediately after aggregation. Samples were centrifuged at 12000 rpm for 30 min to sediment aggregated species; pellet and supernatants were then harvested. Blots were incubated with HRP conjugated anti rmHSP70 antibody and developed by chemiluminescence. Lanes are labelled as follows, M, monomer; A, aggregate; Sup, supernatant; Pel, pellet.