Table 1.

Primers used for qRT-PCR analysis

Primer	Forward primer (5′-3′)	Reverse primer (5′-3′)	TA (°C)
GAPDH	CCTTCCGTGTTCCTAC	GACAACCTGGTCCTCA	60
Shh	AGCCTACAAGCAGTTTATT	TCTTTGCACCTCTGAGTC	56
Ptch1	AATCAGGGGAACTTATCA	ACCGTAAAGGAGGCTTA	56
Smo	CTGACTTTCTGCGTTGC	TTGGGGTTGTCTGTTCG	58
Gli-1	GGATACAACCCAAATGC	TGGCGAATAGACAGAGG	56
Gli-2	TGACCGAAGTGACGATG	AAGTATGGGGAGATGCC	56
Gli-3	CCCCTACATCAACCCAT	CCTGTCAGCAGAGCCAT	58
ZO-1	TGCTCCAGCAGGTCCTAAGT	TGGTAGCTGAGGGCAGAACT	58
Claudin-3	CATCCTGCTGGCCGCCTTCG	CCTGATGATGGTGTTGGCCGAC	56