Fig. 5.
Phosphorylation of ERK in HMRCs and measurement of the concentration of TGF-β1 in supernatants of HRMCs using ELISA. (a–c) Western blot and immunofluorescent analysis in HRMCs transfected with siRNA or miR-155 mimic with or without CXCL13 treatment. (b) Relative expression of CXCR5 to GAPDH was calculated. (c) Immunofluorescent measurement of the tyrosine phosphorylation level in ERK (red). (d) The HRMCs were incubated for 12 h after treatment, and the supernatants were then collected for detecting TGF-β1 levels. Statistical analyses were performed by ANOVA followed by Dunnett’s test. *p < 0.05 (NC versus CXCL13 + NC, miR-155, or siRNA); **p < 0.05 (CXCL13 + NC versus CXCL13 + miR-155 or CXCL13 + siRNA).