Fig. 2.

Growth kinetics of Sardinian ASFV strains in pig and wild-boar monocytes and monocyte-derived macrophages. Pig and wild-boar blood-derived monocytes were infected immediately or differentiated into macrophages (moMΦs). Monocytes and moMΦs were then infected with the virulent historical strain Nu81.2 or the modern strain 22653/14 at an MOI of 0.01. At 0, 24, 48, and 72 hours pi, duplicate samples were collected, and virus levels in culture supernatants were determined by titration. The mean data +/- SD from three independent experiments utilizing three different pigs and three different wild boars are shown. For each cell type, at each time pi, the values for Nu81.2 and 22653/14 were compared using the Mann-Whitney test. ***, p < 0.001; **, p < 0.01; *, p < 0.05