Fig. 2.

Cladogram of the nitrate/nitrite transporters subfamily. The identification of homologs to the nitrate/nitrite putative transporters was performed by searching with BLAST (Altschul et al., 1990) against a non-redundant protein database. The protein data set was aligned using CLUSTAL OMEGA (Sievers et al., 2011). iTOL (Letunic & Bork, 2016) was used for the visualization of the generated tree. Branch-distance is indicated. The proteins used for the comparison are listed in Supplemental Table S1