Skip to main content
. Author manuscript; available in PMC: 2019 Feb 28.
Published in final edited form as: J Neurochem. 2018 Oct 18;147(2):204–221. doi: 10.1111/jnc.14561

Fig. 2.

Fig. 2

Effects of nicotine (0.1–1000 μM) and cotinine (0.5–500 μM) on neuronal viability by 3-(45-dimethylthiazol-2-yl)-25-diphenyltetrazolium bromide (MTT) assay in normoxic (a) and oxygen-glucose deprivation/reoxygenation (OGD/R) conditions (b). In normoxic condition, nicotine at a concentration of 1000 μM significantly decreased neuronal viability compared to no-treatment group. No other nicotine or cotinine concentration caused any significant change in neuronal viability. In OGD/R conditions, nicotine and cotinine, like the normoxic condition, did not cause any significant change in neuronal viability except for 1000 μM nicotine which was significantly toxic to the neurons compared to control. 10 μM nicotine and 5 μM cotinine in combination treatment also did not cause neuronal toxicity in normoxia and OGD/R. *p < 0.05.