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. Author manuscript; available in PMC: 2019 Feb 28.
Published in final edited form as: J Neurochem. 2018 Oct 18;147(2):204–221. doi: 10.1111/jnc.14561

Fig. 5.

Fig. 5

Effects of long-term (5 days) combined nicotine (10 μM) and cotinine (5 μM) treatment on neuronal α7 nAChR expression by immunofluorescence studies. (a) Immunofluorescence images of neurons where green color represents α7 and red color represents the neuronal marker (NeuN). (b) Quantification of α7 expression relative to NeuN. Oxygen-glucose deprivation (OGD) did not cause any change in α7 expression compared to control. OGD/R significantly increased receptor expression compared to normoxia and OGD. Nicotine/cotinine treatment further significantly increased α7 expression in OGD/R condition. Normoxia, OGD, and OGD/R groups are without any nicotine/cotinine exposure, nicotine/cotinine OGD/R group is with 5 days of nicotine/cotinine exposure. *p < 0.05, ***p < 0.001.