KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-pSMAD5 | Abeam | Cat# ab92698; RRID: AB_10561456 |
| Mouse anti-active beta-catenin | Millipore | Cat# 05–665; RRID: AB_309887 |
| Guinea pig anti-Insulin | MP biomedicals | Cat# 651041 |
| Mouse Pan Cytokeratin (AE1/AE3) eFluor 660 | Thermo Fisher Scientific | Cat# 50–9003–82; RRID: AB_2574301 |
| Donkey anti-mouse Fab Alexa488 | Jackson Immuno Research | Cat# 715–547–003; RRID: AB_2340851 |
| Donkey anti-rabbit Alexa488 | Jackson Immuno Research | Cat# 711 −547–003; RRID: AB_2340620 |
| Donkey anti-Guinea pig F(ab”)2 Rhodamine Red X | Jackson Immuno Research | Cat# 706–296–148; RRID: AB_2340469 |
| Guinea Pig polyclonal anti-INS | Acris | Cat# BP5022; RRID: ABJ004211 |
| Mouse monoclonal anti-GCG | Sigma-Aldrich | Cat# G2654; RRID: AB_259852 |
| Sheep anti-BSCL2 antibody | Thermo-Fisher | Cat# PA5–47922; RRID: AB_2606102 |
| Monoclonal Rat anti-TSPAN8 | Thermo-Fisher | Cat# MA5–24179; RRID: AB_2609273 |
| Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse | Sigma-Aldrich | Cat# C2562; RRID: AB_476839 |
| Donkey anti-Rabbit Rhodamine Red-X | Jackson Immuno Research | Cat# 711–297–003; RRID: AB_2340615 |
| Rabbit anti-Nkx6.1 | Sigma-Aldrich | Cat# HPA036774; RRID: AB_10673664 |
| Donkey anti-Guinea pig Alexa 647 | Jackson Immuno Research | Cat# 706–606–148; RRID: AB_2340477 |
| Biological Samples | ||
| Human pancreas sections | University of Iowa Pathology Core | N/A |
| Critical Commercial Assays | ||
| Fluidigm C1 chip | Fluidigm | 100–5760 |
| SMARTer UltraOLow RNA Kit for the Fluidigm C1 | Clontech | 634833 |
| Nextera XT Library Prep Kit | Illumina | FC-131–1096 |
| Mix-n-StainTM CF 488A | Sigma-Aldrich | MX488AS100 |
| Mix-n-StainTM CF 555 | Sigma-Aldrich | MX555S100 |
| Deposited Data | ||
| Our human pancreatic scRNaseq raw and processed data | GEO | GEO: GSE116753 |
| Reanalyzed human pancreatic dataset | GEO | GEO:GSE83139 |
| Reanalyzed human pancreatic dataset | GEO | GEO: GSE81608 |
| Reanalyzed human pancreatic dataset | GEO | GEO: GSE81076 |
| Reanalyzed human pancreatic dataset | GEO | GEO: GSE85241 |
| Reanalyzed human pancreatic dataset | PMID:26691212 | Dataset EV2 |
| Reanalyzed human pancreatic dataset | ArrayExpress | ArrayExpress: E-MTAB-5061 |
| Software and Algorithms | ||
| PyMINEr | This paper https://www.ScienceScott.com/pyminer | N/A |
| FIJI version 1.51w | https://fiji.sc/ | N/A |
| MetaMorph version 7.8.0.0 | https://www.moleculardevices.com | N/A |
| Other | ||
| Ambicon ArrayControl RNAs | Life Technologies | AM1780M |