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Activation particle characterization. (a) Scanning electron microscope (SEM) images of red blood cell, microsphere, and HeLa silica microparticles. (b) Fluorescent and brightfield microscope images of activation particles at 20× magnification. (c) Biotin loading on activation particles quantified by FITC-neutravidin and flow cytometry. (d) Ratiometric loading of anti-CD3ε and anti-CD28 antibodies with fluorescently labeled neutravidin and flow cytometry.
