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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Metabolism. 2018 Mar 29;85:161–170. doi: 10.1016/j.metabol.2018.03.020

Figure 3.

Figure 3

Inflammation marker in kidney of streptozotocin-induced diabetic and control WT mice. (A) Representative images of F4/80 stained kidney. Scale bar = 50μm (B) Quantification of F4/80 positive area in all diabetic groups. (C-F) Quantitative PCR for mouse F4/80 (C), mouse monocyte chemotactic protein-1(MCP-1, D), mouse tumor necrosis factor α (TNF-α, E), mouse inducible nitric oxide synthase (iNOS, F), in kidney. β-actin was used as an internal control. (G) MCP-1 protein in kidney corrected for protein concentration. (A-G, Each diabetic group, n = 9-11. Each control group, n = 4-9). Data represent means ± SEM. *P < 0.05, **P < 0.01 versus the respective diabetes group. # P < 0.05.