Figure 3:

HCs infected with DENV mAb:ZIKV immune complexes result in blunted type I and type III IFN antiviral responses. A) Type I and type III IFN was measured by qRT-PCR at 24 hpi with ZIKV alone (MOI 1 and 10) or mAb:ZIKV (MOI 1) immune complexes. 33.3A06 and EM4CO4 were used at 0.4µg/mL. Representative experiment from n=2 donors. B) Type I and type III IFN was measured in the supernatant at 24 hpi (biological triplicates ±SD). Data were analyzed by 1-way ANOVA and Tukey’s multiple comparison test, *p<0.05, **p<0.01, ***p<0.001. Representative experiment from n=3 donors. Dashed line indicates lower limit of detection. C) Antiviral effector gene expression was measured by qRT-PCR at 24 hpi. Representative experiment from n=2 donors. D) Corresponding protein expression was measured at 24 hpi with the addition of mAb (0.4µg/mL): UV-inactivated ZIKV control (UV-ZIKV; MOI 1) immune complexes. Representative experiment from n=2 donors.