Figure 7:

Targeting FcRn reduces ZIKV infection of human placental explants. A) Human placental explants were treated with 1, 10 or 100 ug/ml of Protein A and subsequently infected ZIKV (5×10⁵ PFU/ml) in the presence of either 33.3A06 IgG1 or IgG3 mAb (0.4 ug/ml). Viral replication was assessed by strand-specific qRT-PCR at 24 hpi (biological triplicates ±SEM). Data were analyzed by 1-way ANOVA and Dunnett’s multiple comparison test, *p<0.05, **p<0.01. Representative experiment from n=3 donors. B) Human placental explants were infected with ZIKV (5×10⁵ PFU/ml) in the presence of either 33.3A06 IgG1 or IgG1-LALA mutant mAbs. Viral replication was assessed by strand-specific qRT-PCR at 24 hpi (biologic triplicates ±SD). Data were analyzed by Student’s t-test, *p<0.05, **p<0.01, Representative experiment from n=4 donors.