Fig. 2. (A) Protein freeze/thaw recovery upon addition of p(ampholyte) following β-Gal storage for 3 days at –20 °C, as % of fresh, unfrozen protein. Concentrations as in Fig. 1, but p(ampholyte) used at 30 mg mL–1. (B) DLS analysis of β-Gal aggregation post freezing compared to fresh protein (black line). (C) β-Gal activity after 1 hour incubation in the cryostorage solutions without freeze/thaw; (D) β-Gal recovery after 4 weeks storage at –80 °C. Error bars are S. D. from minimum of 3 repeats.
