Table 1. Results for TAm catalysed reactions of 18a–26a using phenylethanamine 5, (S)-MBA 7 and IPA 27 as amine donors for pQR2189, pQR2191, pQR2208 and Cv-TAm ^{a , b , c} .

	pQR2189			pQR2191			pQR2208			Cv-TAm
	5	MBA (S)-7	IPA 27	5	MBA (S)-7	IPA 27	5	MBA (S)-7	IPA 27	5	MBA (S)-7
18a		43%	72%		8%	25%		45%	67%		30%
19a		23%	20%		5%	4%		9%	10%		6%
20a		30%	60%		11%	19%		30%	55%		27%
21a		4%	2%		68% d	1%		2%	1%		0%
22a		41%	Quant. e		39%	Quant. e		25%	51%		27%
23a		30%	75%		11%	46%		19%	30%		34%
24a		31%	Quant. e		36%	Quant. e		39%	70%		37%
25a		35%	75% f		3%	13% f		12%	32% f		n.d. g
26a		13%	n.d. g		7%	n.d. g		3%	n.d. g		n.d. g

^aReaction conditions: Amine donor 5 (25 mM), amino acceptor (10 mM), PLP (0.5 mM), KPi buffer pH 7.5 (100 mM) and clarified cell extract (0.4 mg mL^–1), 30 °C, 400 rpm, 18 h. Water was used as a negative control (not shown), red precipitate is indicative of activity. Assay was performed in duplicate.

^bReaction conditions: Amine donor (S)-7 (25 mM), amino acceptor (5 mM), PLP (1 mM), KPi buffer pH 7.5 (100 mM) and clarified cell extract (0.4 mg mL^–1), 30 °C, 400 rpm, 18 h. Acetophenone was detected at 254 nm by HPLC and used to determine percentage conversions. Water was used as a negative control, and any background levels of acetophenone production were subtracted from the reactions. All reactions were performed in triplicate, and standard deviations were <7%.

^cReaction conditions: Amine donor 27 (100 mM), amino acceptor (10 mM), PLP (1 mM), KPi buffer pH 7.5 (100 mM) and clarified cell extract (0.4 mg mL^–1), 35 °C, 400 rpm, 18 h. Yields were determined using HPLC against product standards. All reactions were performed in triplicate, and standard deviations were <5%.

^dBy acetophenone production, product detection confirmed negligible amounts of 21b.

^eQuantitative conversion.

^fYields were determined by 25a depletion, but product presence was confirmed against a product standard.

^gNot determined.