Fig 6. Gmm^WT/Kco_Z flies are significantly more refractory to infection with parasitic trypanosomes and entomopathogenic S. marcescens.

(A) Percentage of Gmm^WT and Gmm^WT/Kco_Z flies infected with T. congolense 14 days after they were co-inoculated with Kco_Z and parasites in their first (teneral) blood meal. (B) Percentage of Gmm^WT and Gmm^WT/Kco_Z flies infected with T. congolense (left graph) and T. brucei (right graph). For these experiments Gmm^WT/Kco_Z flies housed the exogenous bacteria for five days prior to challenge with trypanosomes. In (A) and (B) Statistical analysis was performed using a GLM followed by multiple comparisons and Tukey contrasts, and different letters represents statistical significance between treatments and controls. (C) Kaplan-Meier plot depicting survival of Gmm^WT and Gmm^WT/Kco_Z flies following challenge (three days after administering Kco_Z or Sodalis) with S. marcescens. Infection experiments were performed using three distinct biological replicates (n = 25 flies per replicate). Gmm^WT/Kco_Z flies were also significantly more refractory to S. marcescens infection than were their Gmm^WT counterparts (p = 0.001; log-rank test).