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. 2019 Feb 28;15(2):e1007964. doi: 10.1371/journal.pgen.1007964

Fig 1. Two transcript isoforms of Tiam2 are expressed in spermatogenic cells.

Fig 1

(A) Schematic drawing of the wild type t-complex (+) and the t-haplotype (t). Genes with proven function in TRD are boxed; inversions are indicated by arrows. (B) Northern blot analysis of Tiam2 expression in testes of wild-type and t-haplotype mice. (C) RT-qPCR analysis of Tiam2 transcripts (L is Tiam2l and S is Tiam2s) in testes of C57BL/6 wild-type mice at day 7 to day 24 after birth reflecting the first round of spermatogenesis, and in adult mouse testis. (D) in situ hybridization with a Tiam2 probe. (E) RT-qPCR analysis of Tiam2 transcripts in testis RNA derived from C57BL/6 or from mice carrying t haplotypes on the C57BL/6 background; all values were calculated relative to Tiam2l expression in one C57BL/6 sample set to 1. Abbr.: Mb, Megabases; T, Brachyury; tf, tufted; H-2, mouse MHC complex; Inv(17)1 to Inv(17)4, Inversions 1 to 4 spanning the t-complex; se, Sertoli cell; sg, spermatogonium; sc, spermatocyte; sz, spermatozoa; rs, round spermatid.