Table 1. Proteins identified by nanoLC/MS/MS in fractionated gut fluids from Thermobia domestica with CMCase activity.
The contigs identified in the transcriptome and the matching proteins in the NCBInr database are listed, as well as the normalized quantitative values NSAF in each of the electrophoretic bands analyzed, and the respective glycoside hydrolase family assigned by the CAZy database.
| Contig | Identified protein | Organism | E-Value | Similarity | Accession number | Band 1 NSAF |
Band 2 NSAF |
Band 3 NSAF |
GH family |
|---|---|---|---|---|---|---|---|---|---|
| 23, 309 | endoglucanase | Mastotermes darwiniensis | 0 | 79.9% | CAD54729.1 | 0.33983 | 0.39581 | 0.33043 | GH9 |
| 62 | α-amylase | Blattella germanica | 0 | 78.2% | ABC68516.1 | 0.44423 | 0.06650 | 0.10558 | GH13 |
| 56 | endoglucanase | Panesthia cribrata | 0 | 75.4% | AAF80584.1 | 0.01421 | 0.09824 | 0.12462 | GH9 |
| 359, 444 | Similar to luciferin-regenerating enzyme | Tribolium castaneum | 2.06e-91 | 68.0% | XP_967986.1 | 0.05541 | 0.02064 | 0.01893 | NA |
| 50, 11 | endoglucanase | Coptotermes acinaciformis | 0 | 72.8% | AAK12339.1 | 0.01130 | 0.05306 | 0.02849 | GH9 |
| 9 | endoglucanase | Coptotermes formosanus | 0 | 77.2% | BAB40697.1 | 0 | 0.02094 | 0.02377 | GH9 |
| 253 | endoglucanase | Coptotermes formosanus | 8.38e-17 | 81.8% | ADB12483.1 | 0 | 0.24373 | 0.17412 | GH9 |
| 5 | Chymotrypsin | Teleogryllus emma | 3.69e-76 | 67.6% | ABV32556.1 | 0.02379 | 0.02740 | 0.02474 | NA |
| 4, 25, 488 | Endo-β-1,4-mannanase | Daphnia pulex | 1.23e-132 | 68.1% | EFX71596.1 | 0.02143 | 0.01737 | 0.04125 | GH5 |
| 61 | endo-1,3(4)-β-glucanase | Periplaneta americana | 1.42e-135 | 71.1% | ABR28480.1 | 0 | 0 | 0.022158 | GH16 |
| 65 | Acidic mammalian chitinase | Daphnia pulex | 1.24e-113 | 66.4% | EFX90412.1 | 0.01220 | 0 | 0.01097 | GH18 |
| 234 | endoglucanase | Mastotermes darwiniensis | 3.76e-39 | 76.9% | AAF63725.1 | 0.05048 | 0.03875 | 0.03999 | GH9 |
| 1026 | Similar to β-galactosidase | Tribolium castaneum | 0 | 64.6% | XP_967647.1 | 0.00798 | 0 | 0 | GH35 |
| 68, 54 | Chitinase | Marsupenaeus japonicus | 1.68e-104 | 63.0% | BAA12287.1 | 0.01909 | 0 | 0 | GH18 |
| 157 | Hypothetical protein SINV_01527 | Solenopsis invicta | 3.78e-162 | 65.3% | EFZ09833.1 | 0 | 0 | 0.010978 | GH18 |
| 240 | endoglucanase | Daphnia pulex | 1.00e-13 | 76.8% | EFX80604.1 | 0 | 0 | 0.04438 | GH9 |
| 383 | Chitin deacetylase 9 | Tribolium castaneum | 1.40e-102 | 59.8% | ABW74152.1 | 0 | 0.00437 | 0 | NA |
| 161 | Glucan endo-1,6-β-glucosidase | Haloplasma contractile SSD-17B | 7.54e-90 | 60.0% | EGM33352.1 | 0 | 0.00420 | 0 | GH30 |
| 1785 | Glucan endo-1,6-β-glucosidase | Paenibacillus sp. JDR-2 | 9.89e-34 | 61.3% | ACS99156.1 | 0 | 0.00895 | 0 | GH30 |
| 319 | Midgut trypsin | Glossina morsitans | 9.35e-50 | 57.4% | ADD18692.1 | 0 | 0.00649 | 0 | NA |
| 321 | Actin 5C isoform B | Drosophila melanogaster | 0 | 100.0% | AAN09154.1 | 0 | 0 | 0.00403 | NA |