Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Properties a | Reference or source |
|---|---|---|
| K. pneumoniae Strains | ||
| NTUH-K2044 | K1 serotype, hypermucoviscosity | [23] |
| CG43S3 | CG43 derived strain, rspL mutant (Smr) | [40] |
| CG43S3ΔrcsB | CG43S3 with deletion of rcsB gene | [40] |
| CG43S3ΔrcsC | CG43S3 with deletion of rcsC gene | This study |
| CG43S3ΔrcsD | CG43S3 with deletion of rcsD gene | This study |
| CG43S3ΔrcsF | CG43S3 with deletion of rcsF gene | This study |
| CG43S3ΔyfdX | CG43S3 with deletion of yfdX gene | This study |
| CG43S3ΔhdeB | CG43S3 with deletion of hdeB genes | This study |
| CG43S3ΔhdeB1 | CG43S3 with deletion of hdeB1 gene | This study |
| CG43S3ΔhdeB2 | CG43S3 with deletion of hdeB2 gene | This study |
| CG43S3ΔhdeA | CG43S3 with deletion of hdeA gene | This study |
| CG43S3ΔhdeD | CG43S3 with deletion of hdeD gene | This study |
| CG43S3ΔhdeD1ΔhdeB2 | CG43S3 with deletion of hdeD1, hdeB2 genes | This study |
| CG43S3Δhns | CG43S3 with deletion of hns gene | This study |
| CG43S3ΔlacZ | CG43S3 with deletion lacZ genes | [25] |
| CG43S3ΔlacZΔrcsB | CG43S3ΔrcsB with deletion of lacZ gene | This study |
| E. coli Strains | ||
| JM109 | Cloning host, recA1, supE44, endA1, hsdR17, gyrA96(NalR), relA1, thi-1, Δ(lac-proAB) /F´ [traD36, proAB, laqIqZΔM15] | Laboratory stock |
| S17-1λpir | Bacterial conjugation, Tpr Smr recA, thi, pro, hsdR-M+[PR4-2-Tc::Mu:Kmr Tn7](pir) | Laboratory stock |
| NovaBlue (DE3) | Recombinant protein overexpression host | Laboratory stock |
| Plasmids | ||
| pKAS46 | suicide vector, Kmr Apr | [45] |
| yT&A | cloning vector, Apr | Yeastern Biotech |
| pET30a | His-tag fusion protein expression vector, Kmr | Novagen |
| pET30a-rcsB | rcsB coding region cloned into pET30a, Kmr | This study |
| pET30a-yfdX | yfdX coding region cloned into pET30a, Kmr | This study |
| pRK415 | Broad-host-range IncP plasmid, Tcr | [46] |
| pRK415-yfdX | yfdX complement plasmid, Tcr | This study |
| pRK415-hdeA | hdeA complement plasmid, Tcr | This study |
| pRK415-hdeB | hdeB complement plasmid, Tcr | This study |
| pRK415-hdeD | hdeD complement plasmid, Tcr | This study |
| pRK415-hdeDB | hdeB and hdeD complement plasmid, Tcr | This study |
| pRK415-hdeA-F44A | hdeA F44A complement plasmid, Tcr | This study |
| pRK415-rcsB | rcsB complement plasmid, Tcr | This study |
| pRK415-rcsB-D56A | rcsB D56A complement plasmid, Tcr | This study |
| pRK415-rcsB-D56E | rcsB D56E complement plasmid, Tcr | This study |
| pLacZ15 | A derivative of pYC016, containing a promoterless lacZ from K. pneumoniae CG43S3, Cmr | [26] |
| pLacZ15-PyfdX | yfdX promoter region cloned into pLacZ15 | This study |
| pLacZ15-PhdeDB | hdeDB promoter region cloned into pLacZ15 | This study |
| pLacZ15-PhdeB1 | hdeB1 promoter region cloned into pLacZ15 | This study |
| pLacZ15-PkvhAS | kvhAS promoter region cloned into pLacZ15 | This study |
| pLacZ15-PhdeD1B2 | hdeD1B2 promoter region cloned into pLacZ15 | This study |
| pLacZ15-PhdeA | hdeA promoter region cloned into pLacZ15 | This study |
a Cmr, chloramphenicol resistance; Smr, streptomycin resistance; Kmr, kanamycin resistance.