Fig 7. Association traces of biotin-DNA_ds*Fl binding to SAV and AV.

The $\overline{rF}\left(t\right)$ and $\overline{F}\left(t\right)$ signals of the association reactions of B₇-DNA_ds*Fl (20nM) to (A) AV (520 nM) and (B) SAV (200 nM), at 15°C. (C) Concentration dependence of B₇-DNA_ds*Fl (20 nM) binding to AV at 15°C. All curves (black line) were strongly biphasic. Notice the inversion of SF signals. However, the $\overline{F}\left(t\right)$ traces were in prefect agreement with QY experiments (Table 4).