Table 2. In vitro transfer of the tetK gene from S. saprophyticus to Gram-positive recipient strains.
| Mating organism | Cell count (cfu/ml) | Transfer rate (T/R) | MIC of transconjugant | ||||
|---|---|---|---|---|---|---|---|
| Donor strain | Recipient strain | Donor | Recipient | Transconjuganta | Tet | Lin | |
| S. saprophyticus KM1053 | S. equorum KM1031 | 5.2 × 108 | 3.4 × 108 | 9.5 × 102 | 2.8 × 10−6 | >32 | >512 |
| S. saprophyticus KM1053 | S. equorum KS1039 | 2.5 × 109 | 9.4 × 107 | – | |||
| S. saprophyticus KM1053 | S. aureus USA300 LAC | 1.6 × 109 | 7.3 × 108 | – | |||
| S. saprophyticus KM1053 | E. faecalis OG1RF | 5.0 × 108 | 1.4 × 108 | 5.8 × 103 | 1.2 × 10−5 | >32 | >128 |
Cell counts were repeated three times independently and the mean values of the replicates are presented.
a Transconjugants were confirmed by phenotypic resistance and 16S rRNA gene sequence analysis.
R, recipient; T, transconjugant.