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(A) Immunofluorescent labelling of insulin and ucn3 in zebrafish islets at indicated stages. (B) Quantification of ucn3-positive β-cells in zebrafish islets at the indicated stages in A. n = 6-12 islets per condition. (C) Immunofluorescent labelling of glucagon and ucn3 in zebrafish islets at 72 hpf. (D) Quantification of ucn3-positive α-cells in zebrafish islets at 72 hpf. n = 10 embryos.
